Paraquat Modulates the Differentiation of C2C12 Cells to Myotube
نویسندگان
چکیده
Paraquat is one of herbicides, known to have toxicity in animals and human. It has been reported that paraquat injures lungs and also induces abnormal differentiations in muscle cells. This suggests the possibility that paraquat perturbs the differentiation of muscle. In this study, we examined the effects of paraquat on the differentiation of C2C12 cells, mouse myoblast. In presence of paraquat, C2C12 cells morphologically changed to myotube even in the undifferentiating condition. The amount of myosin heavy chain (MHC), one of the differentiation markers of muscle in C2C12 cells increased with the concentration of paraquat. Because Paraquat produces reactive oxygen species (ROS), effect of ROS on the differentiation of C2C12 cells were examined. The addition of ascorbic acid did not repress the differentiation of C2C12 cells by paraquat. H2O2 also did not induce the differentiation of C2C12 cells. These results suggest that paraquat perturbs the differentiation of C2C12 cells in a ROS-independent manner.
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